Abstract
B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8+ CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8+ CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adjuvants, Immunologic / physiology*
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Animals
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B7 Antigens
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B7-1 Antigen / genetics
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B7-1 Antigen / physiology*
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CHO Cells
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Cell Division / immunology
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Cell Line, Tumor
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Clone Cells
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Cricetinae
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Cytotoxicity Tests, Immunologic
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DNA, Complementary / genetics
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Epitopes, T-Lymphocyte / immunology*
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Lymphocyte Activation / immunology*
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Mast-Cell Sarcoma / immunology*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Nude
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Mice, Transgenic
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T-Lymphocytes, Cytotoxic / cytology*
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T-Lymphocytes, Cytotoxic / immunology*
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Transfection
Substances
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Adjuvants, Immunologic
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B7 Antigens
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B7-1 Antigen
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Cd276 protein, mouse
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DNA, Complementary
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Epitopes, T-Lymphocyte