Identification and expression analysis of EST-based genes in the bud of Lycoris longituba

Yonglan Cui; Xinye Zhang; Yan Zhou; Hong Yu; Lin Tao; Lu Zhang; Jian Zhou; Qiang Zhuge; Youming Cai; Minren Huang

doi:10.1016/s1672-0229(04)02006-6

Identification and expression analysis of EST-based genes in the bud of Lycoris longituba

Genomics Proteomics Bioinformatics. 2004 Feb;2(1):43-6. doi: 10.1016/s1672-0229(04)02006-6.

Authors

Yonglan Cui, Xinye Zhang, Yan Zhou, Hong Yu, Lin Tao, Lu Zhang, Jian Zhou, Qiang Zhuge, Youming Cai, Minren Huang

Abstract

To obtain a primary overview of gene diversity and expression pattern in Lycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were sequenced and 4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967 contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets (totally 1,066 ESTs) had homologues in GenBank and 3,621 ESTs were novel.

Publication types

Comparative Study
Letter
Research Support, Non-U.S. Gov't

MeSH terms

Base Composition
Computational Biology
Expressed Sequence Tags*
Flowering Tops / genetics*
Gene Expression Regulation, Plant*
Genetic Variation*
Lycoris / genetics*