Abstract
We investigated the expression of 50 cytochrome P450 (CYP) genes in the postischemic reperfused rat liver using a DNA microarray. Thirteen CYPs showed absent expression in all experiments and 2 CYPs were induced by pentobarbital, which was used as an anesthetic. Eight of the remaining 35 CYPs showed significantly decreased expression following ischemia-reperfusion. Monitoring CYP expression may be a powerful approach for elucidation of pathways regulating drug metabolism that may be involved in postischemic reperfusion liver injury.
MeSH terms
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Animals
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Aryl Hydrocarbon Hydroxylases / genetics*
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Aryl Hydrocarbon Hydroxylases / metabolism
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Cytochrome P-450 CYP2B1 / genetics*
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Cytochrome P-450 CYP2B1 / metabolism
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Liver / blood supply*
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Male
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Oligonucleotide Array Sequence Analysis
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Phenobarbital / metabolism
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Phenobarbital / pharmacology
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Rats
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Rats, Inbred WKY
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Reperfusion Injury / genetics
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Reperfusion Injury / metabolism
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Reperfusion Injury / physiopathology*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Steroid Hydroxylases / genetics*
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Steroid Hydroxylases / metabolism
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Up-Regulation / drug effects
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Up-Regulation / genetics*
Substances
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Steroid Hydroxylases
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Aryl Hydrocarbon Hydroxylases
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Cytochrome P-450 CYP2B1
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steroid 16-beta-hydroxylase
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Phenobarbital