The resolution of the complete sequences of several hemiascomycete genomes provides new insights into the ways that yeast genomes change in size and in gene contents. These genomes provide evidence of whole-genome duplication occurring before the divergence of Saccharomyces cerevisiae and Candida glabrata, followed by massive gene loss that restored diploidy. The pattern of genome evolution in yeast differs from that in bacteria apparently as a result of stronger selective constraints on bacterial chromosomes.