Abstract
Plants encode subunits for a fourth RNA polymerase (Pol IV) in addition to the well-known DNA-dependent RNA polymerases I, II, and III. By mutation of the two largest subunits (NRPD1a and NRPD2), we show that Pol IV silences certain transposons and repetitive DNA in a short interfering RNA pathway involving RNA-dependent RNA polymerase 2 and Dicer-like 3. The existence of this distinct silencing polymerase may explain the paradoxical involvement of an RNA silencing pathway in maintenance of transcriptional silencing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / enzymology*
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Arabidopsis / genetics
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Base Sequence
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Chromatin / metabolism
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DNA Methylation
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DNA Transposable Elements
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DNA, Plant / genetics*
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DNA, Plant / metabolism
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DNA-Directed RNA Polymerases / chemistry
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DNA-Directed RNA Polymerases / genetics
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DNA-Directed RNA Polymerases / metabolism*
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Gene Silencing*
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Genes, Plant
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Genetic Complementation Test
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Molecular Sequence Data
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Mutation
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Oryza / enzymology
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Oryza / genetics
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Plants, Genetically Modified
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / metabolism
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RNA Interference
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RNA Polymerase II / metabolism
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RNA, Plant / metabolism
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RNA, Small Interfering / metabolism
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Repetitive Sequences, Nucleic Acid
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Transgenes
Substances
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Arabidopsis Proteins
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Chromatin
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DNA Transposable Elements
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DNA, Plant
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Protein Subunits
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RNA, Plant
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RNA, Small Interfering
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Green Fluorescent Proteins
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RNA Polymerase II
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RNA polymerase IV, Arabidopsis
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DNA-Directed RNA Polymerases