Sphingosine-1-phosphate induces early response gene expression in C6 glioma cells

Brain Res Mol Brain Res. 2005 Feb 18;133(2):325-8. doi: 10.1016/j.molbrainres.2004.10.016.

Abstract

Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/mlx24 h) reduced c-fos activation by S1P (100 microM-187+/-6% vs. 411+/-27%) and lysophosphatidic acid (LPA; 100 microM-90+/-34% vs. 188+/-41%), but not by sphingosylphosphorylcholine (SPC; 100 microM-390+/-47% vs. 420+/-44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Line, Tumor
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Estrenes / pharmacology
  • Gene Expression / drug effects*
  • Genes, fos / physiology*
  • Glioma
  • Lysophospholipids / pharmacology*
  • Mice
  • Pertussis Toxin / pharmacology
  • Phosphodiesterase Inhibitors / pharmacology
  • Pyrrolidinones / pharmacology
  • RNA, Messenger / metabolism
  • Receptors, Lysophosphatidic Acid / genetics
  • Receptors, Lysophosphatidic Acid / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sphingosine / analogs & derivatives*
  • Sphingosine / pharmacology*
  • Time Factors

Substances

  • Estrenes
  • Lysophospholipids
  • Phosphodiesterase Inhibitors
  • Pyrrolidinones
  • RNA, Messenger
  • Receptors, Lysophosphatidic Acid
  • 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
  • sphingosine 1-phosphate
  • Pertussis Toxin
  • Sphingosine
  • lysophosphatidic acid