Abstract
Sphingosine-1-phosphate (S1P) caused dose-dependent and time-dependent increases in c-fos mRNA. Pretreatment with pertussis toxin (PTX; 100 ng/mlx24 h) reduced c-fos activation by S1P (100 microM-187+/-6% vs. 411+/-27%) and lysophosphatidic acid (LPA; 100 microM-90+/-34% vs. 188+/-41%), but not by sphingosylphosphorylcholine (SPC; 100 microM-390+/-47% vs. 420+/-44%). RT-PCR analysis and sequencing demonstrated the presence of previously unidentified LPA-responsive Endothelial Differentiation Gene (EDG) receptor mRNAs in C6 cells: EDG-2 and EDG-4.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Blotting, Northern
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Drug Interactions
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Estrenes / pharmacology
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Gene Expression / drug effects*
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Genes, fos / physiology*
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Glioma
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Lysophospholipids / pharmacology*
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Mice
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Pertussis Toxin / pharmacology
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Phosphodiesterase Inhibitors / pharmacology
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Pyrrolidinones / pharmacology
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RNA, Messenger / metabolism
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Receptors, Lysophosphatidic Acid / genetics
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Receptors, Lysophosphatidic Acid / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sphingosine / analogs & derivatives*
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Sphingosine / pharmacology*
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Time Factors
Substances
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Estrenes
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Lysophospholipids
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Phosphodiesterase Inhibitors
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Pyrrolidinones
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RNA, Messenger
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Receptors, Lysophosphatidic Acid
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1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione
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sphingosine 1-phosphate
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Pertussis Toxin
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Sphingosine
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lysophosphatidic acid