The aim of this paper was to obtain allelic frequencies for the 13 CODIS core STR loci (D3S1358, VWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, and D7S820) from autochthonous individuals from Andalusia, Spain. In addition, a number of forensically useful genetic parameters are reported.