Abstract
A cDNA encoding for a predicted small heat shock protein (sHSP), Tsol-sfISP35.6, has been isolated by antibody screening of a Taenia solium c-DNA library. The clone was a full-length sequence (1172 bp) with an open reading frame of 945 bp and encoded for a 314 amino acid protein with deduced molecular mass of 35.6 kDa, isoelectric point of 5.6 arid the characteristic HSP20/alpha-crystallin domain duplicated. It was highly conserved, with a high sequence similarity with other platyhelminth sHSPs. Western blot analysis, using serum from neurocysticercosis patients (NCC), indicated that the purified Tsol-sHSP35.6 expression product was immunogenic, while in indirect ELISA, using the purified Tsol-sHSP35.6 expression product as antigen and serum samples from pigs and humans, 80% of T. solium infected pigs and 84% of patients with active, or 71% of patients with inactive NCC were sero-positive. The possible relevance of Tsol-sHSP35.6 in the diagnosis and pathogenesis of NCC is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Helminth / blood*
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Antibodies, Helminth / immunology
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Antigens, Helminth / chemistry
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Antigens, Helminth / genetics
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Antigens, Helminth / immunology
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Base Sequence
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Blotting, Western
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DNA, Complementary / chemistry
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DNA, Complementary / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Heat-Shock Proteins / chemistry*
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / immunology
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Humans
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Immune Sera / immunology
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Isoelectric Point
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Molecular Sequence Data
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Molecular Weight
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Neurocysticercosis / diagnosis*
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Neurocysticercosis / parasitology
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Open Reading Frames / genetics
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Rabbits
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Sensitivity and Specificity
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Sequence Alignment
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Sequence Homology, Amino Acid
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Swine
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Taenia solium / chemistry*
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Taenia solium / genetics
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Taenia solium / immunology
Substances
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Antibodies, Helminth
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Antigens, Helminth
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DNA, Complementary
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Heat-Shock Proteins
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Immune Sera
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Recombinant Proteins