Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: a cytometric approach

Roberta Ferraresi; Leonarda Troiano; Daniela Rossi; Elena Gualdi; Enrico Lugli; Cristina Mussini; Andrea Cossarizza

doi:10.1016/j.mito.2004.05.015

Mitochondrial membrane potential and nucleosidic inhibitors of HIV reverse transcriptase: a cytometric approach

Mitochondrion. 2004 Jul;4(2-3):271-8. doi: 10.1016/j.mito.2004.05.015.

Authors

Roberta Ferraresi¹, Leonarda Troiano, Daniela Rossi, Elena Gualdi, Enrico Lugli, Cristina Mussini, Andrea Cossarizza

Affiliation

¹ Chair of Immunology, Department of Biomedical Sciences, Section of General Pathology, University of Modena and Reggio Emilia School of Medicine, via Campi, 287, 41100, Modena, Italy.

PMID: 16120391
DOI: 10.1016/j.mito.2004.05.015

Abstract

Mitochondrial toxicity is a relevant side effect of anti-HIV antiretroviral therapy. Adequate experimental models and advanced technologies are crucial to investigate properly mitochondrial toxicity. Functional flow cytometry allows a rapid and sensitive evaluation of several parameters on single cells, and is an excellent tool to investigate the impact of antiviral drug on mitochondrial activity. We used such approach to analyze in vitro mitochondrial toxicity induced by stavudine and zidovudine on cell lines of different origin (hemopoietic: A301, U937, CEM, K562; hepatic: HepG2), and found that the cell lines had a different sensitivity to the action of the drugs.