Abstract
A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Chemical Fractionation / methods
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Emulsions / chemistry
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HIV / genetics*
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HIV / isolation & purification*
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Oils / chemistry*
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RNA, Viral / analysis*
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RNA, Viral / chemistry
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RNA, Viral / genetics*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Water / chemistry*
Substances
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Emulsions
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Oils
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RNA, Viral
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Water