Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M U'Ren; Matthew N Van Ert; James M Schupp; W Ryan Easterday; Tatum S Simonson; Richard T Okinaka; Talima Pearson; Paul Keim

doi:10.1128/JCM.43.11.5771-5774.2005

Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei

J Clin Microbiol. 2005 Nov;43(11):5771-4. doi: 10.1128/JCM.43.11.5771-5774.2005.

Authors

Jana M U'Ren¹, Matthew N Van Ert, James M Schupp, W Ryan Easterday, Tatum S Simonson, Richard T Okinaka, Talima Pearson, Paul Keim

Affiliation

¹ Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA.

Abstract

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

Publication types

Comparative Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Burkholderia mallei / classification*
Burkholderia mallei / genetics
Burkholderia pseudomallei / classification*
Burkholderia pseudomallei / genetics
DNA Primers
Genes, Bacterial / genetics
Geography
Molecular Sequence Data
Nucleotides / genetics
Polymerase Chain Reaction / methods*
Polymorphism, Genetic
Sequence Alignment
Species Specificity
Taq Polymerase

Substances

DNA Primers
Nucleotides
Taq Polymerase