Enzyme stabilizer DTT catalyzes nitrilase analogue hydrolysis of nitriles

Margit Winkler; Anton Glieder; Norbert Klempier

doi:10.1039/b516937b

Enzyme stabilizer DTT catalyzes nitrilase analogue hydrolysis of nitriles

Chem Commun (Camb). 2006 Mar 28:(12):1298-300. doi: 10.1039/b516937b. Epub 2006 Feb 10.

Authors

Margit Winkler¹, Anton Glieder, Norbert Klempier

Affiliation

¹ Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 16/II, A-8010 Graz, Austria.

PMID: 16538253
DOI: 10.1039/b516937b

Abstract

Amides are the dominating products in some nitrilase catalyzed conversions of alpha-activated nitriles, but unexpectedly this hydrolytic reaction is also catalyzed by 1,4-dithio-dl-threitol (DTT), a standard antioxidizing enzyme stabilizer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amides / chemistry*
Aminohydrolases / chemistry*
Catalysis
Dithiothreitol / chemistry*
Enzyme Stability
Hydrolysis
Molecular Structure
Nitriles / chemistry*

Substances

Amides
Nitriles
Aminohydrolases
nitrilase
Dithiothreitol