Hybrid reflecting objectives for functional multiphoton microscopy in turbid media

Opt Lett. 2006 Aug 15;31(16):2447-9. doi: 10.1364/ol.31.002447.

Abstract

Most multiphoton imaging of biological specimens is performed using microscope objectives optimized for high image quality under wide-field illumination. We present a class of objectives designed de novo without regard for these traditional constraints, driven exclusively by the needs of fast multiphoton imaging in turbid media: the delivery of femtosecond pulses without dispersion and the efficient collection of fluorescence. We model the performance of one such design optimized for a typical brain-imaging setup and show that it can greatly outperform objectives commonly used for this task.

Publication types

  • Evaluation Study

MeSH terms

  • Computer-Aided Design
  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Lenses*
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / methods
  • Nephelometry and Turbidimetry / instrumentation*
  • Nephelometry and Turbidimetry / methods
  • Reproducibility of Results
  • Sensitivity and Specificity