Abstract
An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyses extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism*
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Amino Acid Sequence
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Arsenic / metabolism*
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Arsenite Transporting ATPases
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Arsenites*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Genes, Bacterial
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Ion Channels / genetics
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Ion Channels / metabolism*
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Ion Pumps*
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Models, Molecular
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Molecular Sequence Data
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Multienzyme Complexes*
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Nitrogen Fixation / genetics
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Operon
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Sequence Homology, Nucleic Acid
Substances
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Arsenites
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Bacterial Proteins
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Ion Channels
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Ion Pumps
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Multienzyme Complexes
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Adenosine Triphosphatases
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Arsenite Transporting ATPases
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arsenite
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Arsenic