Long-term haematopoietic reconstitution and survival without interleukin-7 in a murine syngeneic bone marrow transplantation model

Scand J Immunol. 2006 Dec;64(6):601-8. doi: 10.1111/j.1365-3083.2006.01847.x.

Abstract

We created a syngeneic mouse bone marrow transplantation (BMT) model to examine the effect of endogenous interleukin-7 (IL-7) on long-term (>or=140 days) haematopoietic reconstitution and survival after BMT. Wild-type (WT) IL-7(+/+) and knockout (KO) IL-7(-/-) mice were lethally irradiated and transplanted with bone marrow. Survival is best (85.7%) in the group WT grafts transplanted into WT recipients (WT-->WT) with a trend towards poorer survival in the other groups (WT-->KO: 60%, KO-->WT: 50%, KO-->KO: 45.5%, differences statistically not significant). If the recipient is deficient for IL-7-producing cells, T- and B-cell reconstitution remain incomplete. If the graft lacks IL-7-producing cells there is a significant delay in T- and NK-cell reconstitution. Interestingly, in the absence of IL-7, T-cell reconstitution is neither delayed nor incomplete because of an expansion of TCRalphabeta(+)/CD4(-)/CD8(-) double negative T cells. Long-term survival and lymphocyte reconstitution after syngeneic BMT can occur despite the absence of IL-7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / chemistry
  • B-Lymphocytes / cytology
  • Bone Marrow Transplantation*
  • CD4 Antigens / analysis
  • CD8 Antigens / analysis
  • Hematopoietic System / cytology
  • Hematopoietic System / physiology
  • Interleukin-7 / deficiency
  • Interleukin-7 / genetics
  • Interleukin-7 / physiology*
  • Killer Cells, Natural / chemistry
  • Killer Cells, Natural / cytology
  • Lymphopoiesis / genetics*
  • Mice / genetics
  • Mice / physiology*
  • Mice, Knockout
  • Models, Animal*
  • Receptors, Antigen, T-Cell, alpha-beta / analysis
  • T-Lymphocytes / chemistry
  • T-Lymphocytes / cytology

Substances

  • CD4 Antigens
  • CD8 Antigens
  • Interleukin-7
  • Receptors, Antigen, T-Cell, alpha-beta