Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens

J Clin Microbiol. 2007 Feb;45(2):581-3. doi: 10.1128/JCM.01823-06. Epub 2006 Nov 22.

Abstract

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Primers
  • DNA, Protozoan / analysis
  • DNA, Protozoan / isolation & purification*
  • Feces / parasitology*
  • Filtration / methods
  • Giardia / classification
  • Giardia / genetics
  • Giardia / growth & development
  • Giardia / isolation & purification*
  • Giardiasis / parasitology*
  • Humans
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal / genetics
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • DNA, Protozoan
  • RNA, Ribosomal