Abstract
The gene encoding a protein of 27 kDa, which is specifically expressed in Vibrio cholerae of serotype Ogawa, was identified and its nucleotide sequence determined. The plasmid carrying this gene was found to convert serotype specificity from Inaba to Ogawa when introduced into the Escherichia coli DH5(pVCI112) cell which harbors a cloned 20-kilobase genomic DNA fragment of V. cholerae NIH35A3 and expresses the 01 antigen of Inaba serotype.
MeSH terms
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Amino Acid Sequence
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Antibodies, Monoclonal / immunology
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Antigens, Bacterial / genetics*
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Antigens, Bacterial / immunology
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Base Sequence
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DNA, Bacterial
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Electrophoresis, Polyacrylamide Gel
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Exons
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Genes, Bacterial
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Molecular Sequence Data
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O Antigens
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Restriction Mapping
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Serotyping
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Vibrio cholerae / classification
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Vibrio cholerae / genetics*
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Vibrio cholerae / immunology
Substances
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Antibodies, Monoclonal
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Antigens, Bacterial
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DNA, Bacterial
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O Antigens
Associated data
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GENBANK/M95211
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GENBANK/X58797
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GENBANK/X58798
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GENBANK/X58799
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GENBANK/X58800
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GENBANK/X58801
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GENBANK/X58802
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GENBANK/X58803
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GENBANK/X58834
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GENBANK/X62841