Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains

Keiko Ishino; Naofumi Tsuchizaki; Jun Ishikawa; Kunimoto Hotta

doi:10.1128/JCM.02099-06

Usefulness of PCR-restriction fragment length polymorphism typing of the coagulase gene to discriminate arbekacin-resistant methicillin-resistant Staphylococcus aureus strains

J Clin Microbiol. 2007 Feb;45(2):607-9. doi: 10.1128/JCM.02099-06. Epub 2006 Dec 13.

Authors

Keiko Ishino¹, Naofumi Tsuchizaki, Jun Ishikawa, Kunimoto Hotta

Affiliation

¹ Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. ishino@nih.go.jp

Abstract

We compared the results of two typing methods for 678 strains of methicillin-resistant Staphylococcus aureus and methicillin-susceptible S. aureus. PCR-restriction fragment length polymorphism typing of the coagulase gene was a more reliable method than coagulase serotyping from the viewpoint of arbekacin resistance.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Aminoglycosides / pharmacology*
Anti-Infective Agents / pharmacology*
Bacterial Typing Techniques
Coagulase / genetics*
Dibekacin / analogs & derivatives*
Dibekacin / pharmacology
Drug Resistance, Bacterial / genetics
Humans
Methicillin Resistance*
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length*
Staphylococcal Infections
Staphylococcus aureus / classification*
Staphylococcus aureus / drug effects*
Staphylococcus aureus / genetics

Substances

Aminoglycosides
Anti-Infective Agents
Coagulase
Dibekacin
arbekacin