Immunocytochemical analysis of the brain and suboesophageal ganglion of the honeybee Apis mellifera L. was combined with Lucifer Yellow backfilling from the corpora cardiaca and intracellular staining of single neurons. It is shown that more than one third of the cells that display FMRFamide-like immunoreactivity (F-LI) project to the corpora cardiaca, suggesting they are neurosecretory. Among the ca. 120 median neurosecretory cells (MNCs) in the pars intercerebralis about 32 show F-LI. The number of immunoreactive MNCs is highly variable and may depend on age and/or diet. Seven of at least 40 lateral neurosecretory cells display F-LI. They project through the brain via the medial branch of the bipartite nervus corporis cardiaci II. In the suboesophageal ganglion three types of immunoreactive neurosecretory cells were identified. Together with the median and the lateral neurosecretory cells in the brain these cells project through a single pair of nerves into the corpora cardiaca suggesting that the nervus corporis cardiaci (NCC) of the honeybee is a fusion of NCC I, II, and III described in other insects.