Regulation of the chitobiose-phosphotransferase system in Vibrio cholerae

Arch Microbiol. 2007 Jun;187(6):433-9. doi: 10.1007/s00203-006-0207-4. Epub 2007 Feb 3.

Abstract

Vibrio cholerae harbours a phosphotransferase system (PTS) enabling the organism to utilise chitosan oligosaccharide, e.g. derived from deacetylated chitin. As shown recently, this utilization system is encoded by the ORFs VC1281-1283 (Meibom et al. in Proc Natl Acad Sci USA, 101:2524-2529, 2004). By using a transcriptional reporter fusion technique, we identified the regulator of the system and characterised gene expression. Furthermore, we found that gene expression of this PTS system is influenced by catabolite regulation and also by an Mlc homologue (VC2007), which in E. coli is a global regulator of sugar metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Chitosan / metabolism
  • Cyclic AMP / genetics
  • Cyclic AMP / metabolism
  • Disaccharides / genetics
  • Disaccharides / metabolism*
  • Escherichia coli Proteins / genetics
  • Gene Expression Regulation, Bacterial*
  • Operon
  • Phosphotransferases / genetics
  • Phosphotransferases / metabolism*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Vibrio cholerae / enzymology*
  • Vibrio cholerae / genetics
  • Vibrio cholerae / metabolism

Substances

  • Bacterial Proteins
  • Disaccharides
  • Escherichia coli Proteins
  • Mlc protein, E coli
  • Repressor Proteins
  • chitobiose
  • Chitosan
  • Cyclic AMP
  • Phosphotransferases