Barks of Pinus massonianm collected from two polluted sites, Qujiang and Xiqiaoshan, and from the relatively clean site Dinghushan were used to evaluate the pollution indication by the determination of their acidity and conductivity. The acidity of the inner and outer barks from the polluted sites was significantly higher than those from the clean site, suggesting that the acidity of the bark occurred in concurrent with the air pollution. The significant lower pH values of the outer bark than the inner bark collected from all sites indicated that the outer bark was more sensitive than the inner bark in response to acid pollution, implying that the outer bark is more preferable when used as indication of atmospheric acid pollution. The conductivities of the inner barks differed significantly among the three sites, with higher values at the clean site. However, the significant differences were not observed among these sites. Furthermore, the pH values for the inner and outer barks were not correlated with the conductivity, which did not coincide with some other studies.