Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene

Appl Environ Microbiol. 2007 Apr;73(7):2354-9. doi: 10.1128/AEM.02603-06. Epub 2007 Feb 9.

Abstract

Species-specific primers and a multiplex PCR assay were developed for the simultaneous identification and differentiation of Pseudomonas fragi, P. lundensis, and P. putida based on the coamplification of different portions of the small subunit of the carbamoyl phosphate synthase gene (carA). The carA multiplex PCR was used to detect the presence of the three Pseudomonas species from beef, chicken, and pork samples and proved to be effective in showing their evolution during the storage of meat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Carbamoyl-Phosphate Synthase (Ammonia) / genetics*
  • DNA Primers
  • Ecology
  • Meat / microbiology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Pseudomonas / genetics
  • Pseudomonas / isolation & purification*
  • Pseudomonas fragi / genetics
  • Pseudomonas fragi / isolation & purification*
  • Pseudomonas putida / genetics
  • Pseudomonas putida / isolation & purification*

Substances

  • DNA Primers
  • Carbamoyl-Phosphate Synthase (Ammonia)

Associated data

  • GENBANK/DQ647052
  • GENBANK/DQ647053
  • GENBANK/EF363547