Abstract
The laccase gene lacD, cloned from a novel laccase-producing basidiomycete Trametes sp. 420, contained 2,052 base pairs (bp) interrupted by 8 introns. lacD displayed a relatively high homology with laccase genes from other white rot fungi, whereas the homology between lacD and laccase genes from plants, insects, or bacteria was less than 25%. A 498-amino acid peptide encoded by the lacD cDNA was heterologously expressed in the Pichia pastoris strain GS115, resulting in the highest yield of laccase (8.3 x 10(4) U/l) as determined with ABTS (2,2'-azinobis [3-ethylbenzothia-zoline-6-sulfonic acid]) as the substrate. Additionally, the enzyme activity of recombinant laccase on decolorization of some industrial dyes was assessed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anthraquinones / metabolism
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Benzothiazoles / metabolism
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Cloning, Molecular
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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Evans Blue / metabolism
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Fungal
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Indigo Carmine
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Indoles / metabolism
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Laccase / genetics*
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Laccase / metabolism
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Molecular Sequence Data
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Pichia / genetics*
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Polyporales / enzymology
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Polyporales / genetics*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Sequence Analysis, DNA
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Substrate Specificity
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Sulfonic Acids / metabolism
Substances
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Anthraquinones
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Benzothiazoles
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DNA, Fungal
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Fungal Proteins
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Indoles
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Recombinant Proteins
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Sulfonic Acids
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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Evans Blue
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Indigo Carmine
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Laccase