The Drosophila SET domain encoding gene dEset is essential for proper development

Hereditas. 2006 Dec;143(2006):177-88. doi: 10.1111/j.2006.0018-0661.01970.x.

Abstract

We have identified dEset, the fly homolog of human SETDB1 and mouse ESET histone lysine methyltransferases (HKMTases) that methylates the lysine 9 residue of histone 3 (H3-K9) and negatively regulates transcription of target genes. By using spatio-temporal RNA interference we show that dEset is required at several stages of development coinciding with ecdysone pulses, possibly as a repressor of transcription of target genes. Several interacting partners, for example USP, spire, and cut up were identified in a yeast two-hybrid screen. The spatio-temporal expression profiles of dEset and its potential partners suggest that they may act together or even in a larger complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Body Patterning / genetics
  • Drosophila / enzymology
  • Drosophila / genetics
  • Drosophila / growth & development*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Gene Expression Regulation, Developmental
  • Histone-Lysine N-Methyltransferase / genetics
  • Histone-Lysine N-Methyltransferase / physiology*
  • Male
  • Mice
  • Models, Genetic
  • Molecular Sequence Data
  • Phenotype
  • Protein Methyltransferases / genetics
  • Protein Structure, Tertiary
  • RNA Interference
  • Two-Hybrid System Techniques

Substances

  • Drosophila Proteins
  • Protein Methyltransferases
  • Histone-Lysine N-Methyltransferase
  • SETDB1 protein, mouse
  • egg protein, Drosophila