Influence of disk preparation on detection of metallo-beta-lactamase-producing isolates by the combined disk assay

J Clin Microbiol. 2007 Jun;45(6):2058-60. doi: 10.1128/JCM.02467-06. Epub 2007 Apr 4.

Abstract

The combined disk assay has been used for detection of metallo-beta-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many beta-lactam/metallo-beta-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 beta-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agar
  • Anti-Bacterial Agents / pharmacology*
  • Carbapenems / pharmacology
  • Ceftazidime / pharmacology*
  • Culture Media
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / enzymology
  • Gram-Negative Bacteria / isolation & purification
  • Humans
  • Imipenem / pharmacology*
  • Microbial Sensitivity Tests / instrumentation*
  • Microbial Sensitivity Tests / methods
  • beta-Lactam Resistance
  • beta-Lactamase Inhibitors
  • beta-Lactamases / biosynthesis*

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • Culture Media
  • beta-Lactamase Inhibitors
  • Imipenem
  • Agar
  • Ceftazidime
  • beta-Lactamases