A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum

M L Reyes Escogido; A De León Rodríguez; A P Barba de la Rosa

doi:10.1007/s10529-007-9376-8

A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum

Biotechnol Lett. 2007 Aug;29(8):1249-53. doi: 10.1007/s10529-007-9376-8. Epub 2007 May 9.

Authors

M L Reyes Escogido¹, A De León Rodríguez, A P Barba de la Rosa

Affiliation

¹ Division of Molecular Biology, Institute for Scientific and Technological Research of San Luis Potosí, SLP, México, Camino a la Presa San José 2055, Col Lomas 4 sección, CP 78216, San Luis Potosi, SLP, Mexico.

PMID: 17487549
DOI: 10.1007/s10529-007-9376-8

Abstract

A novel expression vector (pLR) driven by hup promoter and Bifidobacterium beta-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Bifidobacterium / metabolism*
Biotechnology / methods*
Codon
Escherichia coli / metabolism*
Gene Expression Regulation
Genetic Vectors
Humans
Interleukin-10 / chemistry
Interleukin-10 / metabolism
Interleukin-10 / physiology*
Molecular Sequence Data
Plasmids / metabolism
Promoter Regions, Genetic
Recombinant Proteins / chemistry

Substances

Codon
Recombinant Proteins
Interleukin-10