Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line

J Proteome Res. 2007 Jul;6(7):2550-64. doi: 10.1021/pr060701g. Epub 2007 Jun 9.

Abstract

Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which G beta2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Nucleus / chemistry
  • Chromatography, Liquid
  • Computational Biology
  • Cytosol / chemistry
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Neoplasm Proteins / analysis*
  • Neuroblastoma / chemistry
  • Neuroblastoma / metabolism*
  • Peripheral Nervous System Neoplasms / chemistry
  • Peripheral Nervous System Neoplasms / metabolism*
  • Protein Array Analysis*
  • Proteomics*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tretinoin / pharmacology

Substances

  • Neoplasm Proteins
  • Tretinoin