Unifying thermodynamic and kinetic descriptions of single-molecule processes: RNA unfolding under tension

J Phys Chem B. 2007 Aug 16;111(32):9598-602. doi: 10.1021/jp073413w. Epub 2007 Jul 21.

Abstract

We use mesoscopic nonequilibrium thermodynamics theory to describe RNA unfolding under tension. The theory introduces reaction coordinates, characterizing a continuum of states for each bond in the molecule. The unfolding considered is so slow that one can assume local equilibrium in the space of the reaction coordinates. In the quasi-stationary limit of high sequential barriers, our theory yields the master equation of a recently proposed sequential-step model. Nonlinear switching kinetics is found between open and closed states. Our theory unifies the thermodynamic and kinetic descriptions and offers a systematic procedure to characterize the dynamics of the unfolding process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Kinetics
  • Models, Biological
  • Nucleic Acid Denaturation
  • RNA / chemistry*
  • Stress, Mechanical
  • Thermodynamics*

Substances

  • RNA