Abstract
Single intradermal or intramuscular inoculations of GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus (SHIV)-89.6 vaccine, which consists of DNA priming followed by modified vaccinia Ankara (MVA) boosting, increased protection of both the blood and intestines against the acute phase of an intrarectal SHIV-89.6P challenge. GM-CSF appeared to contribute to protection by enhancing two antibody responses: the avidity maturation of anti-Env IgG in blood (p=or<0.01) and the presence of long lasting anti-viral IgA in rectal secretions (p<0.01). The avidity of anti-Env IgG showed strong correlations with protection both pre and post challenge. Animals with the highest avidity anti-Env Ab had 1000-fold reductions in peak viremia over those with the lowest avidity anti-Env Ab. The enhanced IgA response was associated with the best protection, but did not achieve significance.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
AIDS Vaccines / genetics
-
AIDS Vaccines / immunology*
-
Adjuvants, Immunologic / administration & dosage*
-
Adjuvants, Immunologic / genetics
-
Animals
-
Antibodies, Viral / immunology*
-
Antibody Affinity / immunology
-
Granulocyte-Macrophage Colony-Stimulating Factor / genetics
-
Granulocyte-Macrophage Colony-Stimulating Factor / immunology*
-
Immunization, Secondary
-
Immunoglobulin A / analysis
-
Immunoglobulin A / immunology
-
Immunoglobulin G / blood
-
Immunoglobulin G / immunology
-
Injections, Intradermal
-
Injections, Intramuscular
-
Macaca mulatta
-
Rectum / immunology*
-
SAIDS Vaccines / genetics
-
SAIDS Vaccines / immunology*
-
Simian Acquired Immunodeficiency Syndrome / drug therapy
-
Simian Acquired Immunodeficiency Syndrome / prevention & control
-
Vaccines, DNA / genetics
-
Vaccines, DNA / immunology*
-
Vaccinia virus / genetics
-
Vaccinia virus / immunology
-
Viral Load
-
Viral Vaccines / genetics
-
Viral Vaccines / immunology
-
Viremia / immunology
Substances
-
AIDS Vaccines
-
Adjuvants, Immunologic
-
Antibodies, Viral
-
Immunoglobulin A
-
Immunoglobulin G
-
SAIDS Vaccines
-
Vaccines, DNA
-
Viral Vaccines
-
Granulocyte-Macrophage Colony-Stimulating Factor