Abstract
We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Child, Preschool
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Cytomegalovirus / genetics
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Cytomegalovirus / isolation & purification*
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Cytomegalovirus Infections / congenital*
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Cytomegalovirus Infections / diagnosis*
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DNA, Viral / blood*
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Humans
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Infant
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Infant, Newborn
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Polymerase Chain Reaction / methods*
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Retrospective Studies
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Sensitivity and Specificity
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Viral Load