Abstract
We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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DNA / immunology
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DNA-Binding Proteins / immunology
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Humans
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Immunoassay / methods*
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Microchemistry / methods*
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Polymerase Chain Reaction / methods*
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Prostate-Specific Antigen / analysis*
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Prostate-Specific Antigen / immunology*
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Recombinant Proteins / immunology
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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DNA-Binding Proteins
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Recombinant Proteins
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DNA
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Prostate-Specific Antigen