Biosensor for total cholesterol estimation using N-(2-aminoethyl)-3-aminopropyltrimethoxysilane self-assembled monolayer

Anal Bioanal Chem. 2007 Dec;389(7-8):2235-42. doi: 10.1007/s00216-007-1655-7. Epub 2007 Nov 6.

Abstract

Cholesterol oxidase (ChOx), cholesterol esterase (ChEt), and horseradish peroxidase (HRP) have been co-immobilized covalently on a self-assembled monolayer (SAM) of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (AEAPTS) deposited on an indium-tin-oxide (ITO) glass surface. These enzyme-modified (ChOx-ChEt-HRP/AEAPTS/ITO) biosensing electrodes have been used to estimate cholesteryl oleate from 10 to 500 mg dL(-1). The sensitivity, Km value, and shelf-life of these ChEt-ChOx-HRP/AEAPTS/ITO biosensing electrodes have been found to be 124 nA mg(-1) dL, 95.098 mg dL(-1) (1.46 mmol L(-1)), and ten weeks, respectively. The ChEt-ChOx-HRP/AEAPTS/ITO bio-electrodes have been used to estimate total cholesterol in serum samples.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques* / instrumentation
  • Cholesterol / blood*
  • Enzymes, Immobilized / chemistry
  • Humans
  • Molecular Structure
  • Propylamines / chemistry*
  • Silanes / chemistry*
  • Silicone Elastomers
  • Surface Properties

Substances

  • Enzymes, Immobilized
  • Propylamines
  • Silanes
  • Silicone Elastomers
  • aminoethyl-aminopropyl-trimethoxysilane
  • Cholesterol