Abstract
A novel ferritin cDNA, SferH-5, has been cloned from 7-day-old soybean seedlings. Putative SferH-5 has 96% identity with SferH-1 reported previously. All the five amino acid variants distributed in the mature region are not involved in highly conserved residues associated with ferroxidase activity center. We speculate that SferH-5 encodes a novel 26.5-kDa subunit of soybean seed ferritin, which is designated H-5 in this study. Recombinant H-5 was able to assemble, together with co-expressed H-2, as a functional soybean seed ferritin-like complex, H-5/H-2. Our data reveal the potential heterogeneity of the 26.5-kDa subunit of soybean seed ferritin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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Ferritins / genetics*
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Ferritins / metabolism
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Gene Expression Regulation, Plant
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Genes, Plant / genetics*
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Genetic Variation*
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Genetic Vectors
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Glycine max / genetics*
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Iron / metabolism
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Kinetics
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Molecular Sequence Data
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Molecular Weight
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Protein Subunits / genetics*
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Seeds / embryology
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Seeds / genetics*
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Sequence Alignment
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Transcription, Genetic
Substances
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Plant Proteins
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Protein Subunits
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Recombinant Proteins
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Ferritins
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Iron