Abstract
Bacterial chromosomes (though not Escherichia coli and some other gamma-proteobacterial chromosomes) contain parS sequences and parAB genes encoding partitioning proteins, i.e. ParA (ATPase) and ParB (DNA-binding proteins) that are components of the segregation machinery. Here, mycobacterial parABS elements were characterized for the first time. parAB genes are not essential in Mycobacterium smegmatis; however, elimination or overexpression of ParB protein causes growth inhibition. Deletion of parB also leads to a rather severe chromosome segregation defect: up to 10% of the cells were anucleate. Mycobacterial ParB protein uses three oriC-proximal parS sequences as targets to organize the origin region into a compact nucleoprotein complex. Formation of such a complex involves ParB-ParB interactions and is assisted by ParA protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence
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Chromosome Segregation*
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Chromosomes, Bacterial / genetics*
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Culture Media
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Deletion
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Gene Expression Regulation, Bacterial
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Molecular Sequence Data
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Mycobacterium smegmatis / genetics*
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Mycobacterium smegmatis / growth & development
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Mycobacterium smegmatis / metabolism
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / metabolism
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Operon
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Replication Origin
Substances
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Bacterial Proteins
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Culture Media
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DNA-Binding Proteins
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Recombinant Fusion Proteins
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Adenosine Triphosphatases