Abstract
The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to selectively bind its cognate RNA, and it demonstrates that the conserved beta-sheet surface is a flexible architecture that can evolve to recognize diverse RNA hairpins.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriophages / chemistry
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Bacteriophages / genetics
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Binding Sites
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Crystallography, X-Ray
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Evolution, Molecular
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Models, Molecular
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Nucleic Acid Conformation
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Protein Conformation
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RNA Viruses / genetics
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RNA Viruses / metabolism
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RNA, Viral / chemistry*
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RNA, Viral / genetics
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RNA, Viral / metabolism*
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Viral Proteins / chemistry*
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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RNA, Viral
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Viral Proteins