Structural basis for the coevolution of a viral RNA-protein complex

Nat Struct Mol Biol. 2008 Jan;15(1):103-5. doi: 10.1038/nsmb1327. Epub 2007 Dec 9.

Abstract

The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to selectively bind its cognate RNA, and it demonstrates that the conserved beta-sheet surface is a flexible architecture that can evolve to recognize diverse RNA hairpins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / chemistry
  • Bacteriophages / genetics
  • Binding Sites
  • Crystallography, X-Ray
  • Evolution, Molecular
  • Models, Molecular
  • Nucleic Acid Conformation
  • Protein Conformation
  • RNA Viruses / genetics
  • RNA Viruses / metabolism
  • RNA, Viral / chemistry*
  • RNA, Viral / genetics
  • RNA, Viral / metabolism*
  • Viral Proteins / chemistry*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • RNA, Viral
  • Viral Proteins

Associated data

  • PDB/2QUD
  • PDB/2QUX