Cost-effective method of DNA extraction from taeniid eggs

Parasitol Res. 2008 Mar;102(4):811-3. doi: 10.1007/s00436-007-0855-6. Epub 2008 Jan 3.

Abstract

A new cost-effective method using silicon dioxide- and guanidine isothiocyanate-containing buffers, after previous alkaline lysis, was established for the DNA extraction from taeniid eggs isolated from canine faeces. The purified DNA can be used to amplify the species-specific 12S mitochondrial DNA of Echinococcus multilocularis in direct and nested polymerase chain reaction in order to differentiate between E. multilocularis and Taenia spp.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • DNA, Helminth / analysis
  • DNA, Helminth / isolation & purification*
  • Dog Diseases / diagnosis*
  • Dog Diseases / parasitology
  • Dogs
  • Echinococcosis / diagnosis
  • Echinococcosis / parasitology
  • Echinococcosis / veterinary
  • Echinococcus multilocularis / classification
  • Echinococcus multilocularis / genetics
  • Echinococcus multilocularis / growth & development
  • Echinococcus multilocularis / isolation & purification*
  • Feces / parasitology
  • Molecular Sequence Data
  • Ovum / parasitology*
  • Parasite Egg Count
  • Polymerase Chain Reaction / economics*
  • Polymerase Chain Reaction / methods
  • Species Specificity
  • Taenia / classification
  • Taenia / genetics
  • Taenia / growth & development
  • Taenia / isolation & purification*
  • Taeniasis / diagnosis
  • Taeniasis / parasitology
  • Taeniasis / veterinary

Substances

  • DNA, Helminth

Associated data

  • GENBANK/EU043371
  • GENBANK/EU043772
  • GENBANK/EU043773