Abstract
A new cost-effective method using silicon dioxide- and guanidine isothiocyanate-containing buffers, after previous alkaline lysis, was established for the DNA extraction from taeniid eggs isolated from canine faeces. The purified DNA can be used to amplify the species-specific 12S mitochondrial DNA of Echinococcus multilocularis in direct and nested polymerase chain reaction in order to differentiate between E. multilocularis and Taenia spp.
MeSH terms
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Animals
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DNA, Helminth / analysis
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DNA, Helminth / isolation & purification*
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Dog Diseases / diagnosis*
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Dog Diseases / parasitology
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Dogs
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Echinococcosis / diagnosis
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Echinococcosis / parasitology
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Echinococcosis / veterinary
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Echinococcus multilocularis / classification
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Echinococcus multilocularis / genetics
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Echinococcus multilocularis / growth & development
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Echinococcus multilocularis / isolation & purification*
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Feces / parasitology
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Molecular Sequence Data
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Ovum / parasitology*
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Parasite Egg Count
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Polymerase Chain Reaction / economics*
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Polymerase Chain Reaction / methods
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Species Specificity
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Taenia / classification
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Taenia / genetics
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Taenia / growth & development
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Taenia / isolation & purification*
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Taeniasis / diagnosis
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Taeniasis / parasitology
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Taeniasis / veterinary
Associated data
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GENBANK/EU043371
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GENBANK/EU043772
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GENBANK/EU043773