Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

Izuho Hatada; Sumiyo Morita; Mika Kimura; Takuro Horii; Riu Yamashita; Kenta Nakai

doi:10.1007/s10038-007-0228-0

Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

J Hum Genet. 2008;53(2):185-191. doi: 10.1007/s10038-007-0228-0. Epub 2008 Jan 17.

Authors

Izuho Hatada¹, Sumiyo Morita², Mika Kimura², Takuro Horii², Riu Yamashita³, Kenta Nakai³

Affiliations

¹ Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Japan. ihatada@showa.gunma-u.ac.jp.
² Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Japan.
³ Laboratory of Functional Analysis in silico, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.

PMID: 18200438
DOI: 10.1007/s10038-007-0228-0

Abstract

Epigenetic regulation including DNA methylation plays an important role in several differentiation processes. We profiled global DNA methylation in the neural differentiation of P19 embryonic carcinoma cells using a microarray-based method called MIAMI. We found a genome-wide demethylation of genes. This suggests demethylation rather than methylation is important in neural differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / genetics*
DNA Methylation*
Embryonal Carcinoma Stem Cells / pathology*
Gene Expression Profiling
Genome / physiology*
Mice
Neurons / metabolism
Neurons / pathology*
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured

Substances

RNA, Messenger