Use of real-time PCR to detect Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in the saliva and salivary glands of haemoplasma-infected cats

Rachel S Dean; Chris R Helps; Timothy J Gruffydd Jones; Séverine Tasker

doi:10.1016/j.jfms.2007.12.007

Use of real-time PCR to detect Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in the saliva and salivary glands of haemoplasma-infected cats

J Feline Med Surg. 2008 Aug;10(4):413-7. doi: 10.1016/j.jfms.2007.12.007. Epub 2008 Mar 3.

Authors

Rachel S Dean¹, Chris R Helps, Timothy J Gruffydd Jones, Séverine Tasker

Affiliation

¹ School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK. rachel.dean@aht.org.uk

PMID: 18313962
DOI: 10.1016/j.jfms.2007.12.007

Abstract

Feline haemoplasma infection can cause haemolytic anaemia. The natural method of transmission of haemoplasmas between cats is currently unknown but the nature of some of the risk factors for infection suggests that saliva may act as a mode of transmission. The aim of this study was to determine if Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (CMhm) DNAs could be amplified from saliva and salivary gland samples collected from haemoplasma-infected cats.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cat Diseases / diagnosis*
Cat Diseases / transmission
Cats
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Gene Amplification
Mycoplasma / isolation & purification*
Mycoplasma Infections / diagnosis
Mycoplasma Infections / transmission
Mycoplasma Infections / veterinary*
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / veterinary*
Saliva / microbiology*
Salivary Glands / microbiology*
Sensitivity and Specificity
Species Specificity
Time Factors

Substances

DNA, Bacterial