Low-frequency electromagnetic field effects on functional groups in human skin keratinocytes cells revealed by IR-SNOM

J Microsc. 2008 Mar;229(Pt 3):551-4. doi: 10.1111/j.1365-2818.2008.01942.x.

Abstract

Human HaCaT cells, exposed for 24 h to a 1 mT (rms) 50 Hz sinusoidal magnetic field in a temperature-regulated solenoid, suffer detectable changes in their biochemical properties and shapes. By using infrared wavelength-selective scanning near-field optical microscopy, we observed changes in the distribution of the inner chemical functional groups and in the cell morphology with a resolution of 80-100 nm.

MeSH terms

  • Cell Line
  • Electromagnetic Fields*
  • Humans
  • Infrared Rays
  • Keratinocytes* / metabolism
  • Keratinocytes* / radiation effects
  • Keratinocytes* / ultrastructure
  • Microscopy, Scanning Probe
  • Skin / cytology*