Abstract
The arsR gene encodes the regulatory protein of the plasmid-encoded arsenical resistance operon. A series of in-frame fusions was constructed between the C-terminally truncated arsR gene and the coding region for the mature form of beta-lactamase (blaM). Fusions containing most of the arsR gene were still inducible by arsenicals. Fusions containing less than 102 residues of the 117-residue ArsR protein were constitutive. When a wild-type arsR gene was placed in trans, the constitutive constructs were again inducible. The results demonstrate that the ArsR protein is a trans-acting regulatory protein which controls its own expression.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Amino Acid Sequence
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Arsenic / pharmacology
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Arsenite Transporting ATPases
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Arsenites*
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Bacterial Proteins*
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Escherichia coli / drug effects
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial / drug effects
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Gene Expression Regulation, Bacterial / genetics*
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Genes, Regulator / genetics
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Immunoblotting
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Ion Pumps*
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Molecular Sequence Data
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Multienzyme Complexes*
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Operon / genetics
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R Factors / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Trans-Activators / genetics*
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Transcription Factors / genetics*
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beta-Lactamases / genetics
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beta-Lactamases / metabolism
Substances
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ArsR protein, E coli
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Arsenites
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Bacterial Proteins
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Escherichia coli Proteins
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Ion Pumps
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Multienzyme Complexes
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Recombinant Fusion Proteins
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Trans-Activators
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Transcription Factors
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beta-Lactamases
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Adenosine Triphosphatases
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Arsenite Transporting ATPases
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arsenite
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Arsenic