Evaluation of new commercial real-time PCR quantification assay for prenatal diagnosis of cytomegalovirus congenital infection

Aurélie Ducroux; Samira Cherid; Alexandra Benachi; Yves Ville; Marianne Leruez-Ville

doi:10.1128/JCM.02179-07

Evaluation of new commercial real-time PCR quantification assay for prenatal diagnosis of cytomegalovirus congenital infection

J Clin Microbiol. 2008 Jun;46(6):2078-80. doi: 10.1128/JCM.02179-07. Epub 2008 Apr 16.

Authors

Aurélie Ducroux¹, Samira Cherid, Alexandra Benachi, Yves Ville, Marianne Leruez-Ville

Affiliation

¹ Laboratoire de Virologie, Hôpital Necker-Enfants-malades, 75015 Paris, France.

Abstract

A new commercial real-time human cytomegalovirus (HCMV) PCR kit was evaluated after automated DNA extraction of 153 amniotic fluids in parallel with an in-house real-time PCR assay. The commercial kit displayed 100% sensitivity/specificity compared to the "in-house" assay and was suitable for prenatal diagnosis of HCMV congenital infection.

Publication types

Evaluation Study

MeSH terms

Amniotic Fluid / virology*
Cytomegalovirus / genetics
Cytomegalovirus / isolation & purification*
Cytomegalovirus Infections / congenital
Cytomegalovirus Infections / diagnosis*
Cytomegalovirus Infections / virology
DNA, Viral / analysis
DNA, Viral / isolation & purification
Female
Fetal Diseases / diagnosis*
Fetal Diseases / virology
Humans
Polymerase Chain Reaction / methods*
Pregnancy
Pregnancy Complications, Infectious / diagnosis
Pregnancy Complications, Infectious / virology
Prenatal Diagnosis / methods*
Reagent Kits, Diagnostic
Sensitivity and Specificity

Substances

DNA, Viral
Reagent Kits, Diagnostic