Detection of mutations by denaturing gradient gel electrophoresis

Curr Protoc Hum Genet. 2001 May:Chapter 7:Unit 7.5. doi: 10.1002/0471142905.hg0705s17.

Abstract

This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels.

MeSH terms

  • DNA / genetics
  • DNA / isolation & purification
  • DNA Mutational Analysis / methods*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Genetics, Medical
  • Humans
  • Nucleic Acid Denaturation

Substances

  • DNA