In an attempt to know the potential risk of human disease after exposure to ticks in La Rioja (North of Spain), the objective of our study was to investigate the presence of Rickettsia species in Haemaphysalis ticks collected in our area. A total of 177 Haemaphysalis spp. belonging to three species (H. punctata, H. sulcata, and H. inermis) were subjected to DNA extraction and tested by polymerase chain reaction (PCR) targeting three rickettsial genes: gltA, ompB, and ompA. Six (3 H. inermis, 2 H. punctata, and 1 H. sulcata) of the 177 specimens were found to be infected (3.4%). The rickettsiae in H. inermis ticks (n = 3) were identified as Rickettsia aeschlimannii by sequencing of the genes coding for gltA, ompB, and ompA. Nucleotide sequences from H. punctata and H. sulcata samples that yielded PCR products (n = 3), showed >99% similarity with sequences of Rickettsia endosymbiont of H. sulcata and 'Candidatus Rickettsia hoogstraalii' for gltA and ompB genes, respectively. Attempts to amplify ompA from these two H. punctata and one H. sulcata failed. This study suggests that H. inermis could be a vector for tick-borne spotted fever caused by R. aeschlimannii in the north of Spain. Further studies on characterization and culture of rickettsial endosymbionts found in Haemaphysalis spp. should be performed.