Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat

N N Veiko; S V Kostyuk; A V Ermakov; E A Kalashnikova; O A Kupavtseva; T A Ryazantseva; A I Speranskii

doi:10.1007/s10517-007-0317-2

Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat

Bull Exp Biol Med. 2007 Sep;144(3):304-8. doi: 10.1007/s10517-007-0317-2.

Authors

N N Veiko¹, S V Kostyuk, A V Ermakov, E A Kalashnikova, O A Kupavtseva, T A Ryazantseva, A I Speranskii

Affiliation

¹ Medical and Genetic Research Center, Russian Academy of Medical Sciences. ribgene@rambler.ru

PMID: 18457021
DOI: 10.1007/s10517-007-0317-2

Abstract

Enzyme immunoassay showed that blood serum from healthy donors contains specific high-affinity antibodies (apparent association constant > or = 5 x 10(9) M(-1)) against a fragment of transcribed region of ribosomal DNA repeat of human serum, which are present in a free form or are bound to extracellular DNA. Preheating of the serum at 55 degrees C and high ionic strength (1.5 M NaCl) had no effect on the interaction of antibodies with this fragment. Competitive binding assay showed that these antibodies recognize DNA epitopes, which differ from the epitopes recognized by most anti-DNA antibodies in systemic lupus erythematosus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Animals
Antibodies, Antinuclear / blood*
DNA, Ribosomal / immunology*
Female
Humans
Serum / immunology*

Substances

Antibodies, Antinuclear
DNA, Ribosomal