Detection of lectin-glycan interaction using high resolution surface plasmon resonance

Kyle J Foley; Erica S Forzani; Lokesh Joshi; Nongjian Tao

doi:10.1039/b719321a

Detection of lectin-glycan interaction using high resolution surface plasmon resonance

Analyst. 2008 Jun;133(6):744-6. doi: 10.1039/b719321a. Epub 2008 Mar 12.

Authors

Kyle J Foley¹, Erica S Forzani, Lokesh Joshi, Nongjian Tao

Affiliation

¹ Department of Electrical Engineering, Arizona State University, Tempe, AZ 85282-5706, USA.

PMID: 18493673
DOI: 10.1039/b719321a

Abstract

We report the real-time and label-free detection of direct disaccharide binding to a lectin using a differential surface plasmon resonance detection method that allows for measurement of nanomolar concentrations of disaccharides.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Lectins / chemistry*
Nanotechnology
Polysaccharides / chemistry*
Protein Binding
Surface Plasmon Resonance / methods*

Substances

Lectins
Polysaccharides