Four new 26-hydroxylated phytoecdysteroids, 2-deoxy-5,20,26-trihydroxyecdysone (1), 5,20,26-trihydroxyecdysone 20,22-acetonide (2), 2-deoxy-5,20,26-trihydroxyecdysone 20,22-acetonide (3), and 20,26-dihydroxyecdysone 20,22-acetonide (4), were isolated from the herb Silene viridiflora, and their structures were elucidated by means of one- and two-dimensional NMR and mass spectrometry.