Label-free, all-electrical, in situ human epidermal growth receptor 2 detection

Rev Sci Instrum. 2008 Jul;79(7):076101. doi: 10.1063/1.2949831.

Abstract

Using 3-mercaptopropyltrimethoxysilane (MPS)-coated (PbMg 1/3 Nb 2/3 O3)0.63-(PbTiO3)0.37 (PMN-PT)/tin and lead zirconate titanate/glass piezoelectric microcantilever sensors (PEMSs) with single-chain variable fragment (scFv) immobilized on the MPS surface, we have demonstrated real-time, label-free detection of human epidermal growth factor receptor 2 (Her2) in a background of 1 mg/ml bovine serum albumin. Coupled with a scFv with a KD of 3.4 x 10(-8)M, the MPS-insulated PMN-PT/tin PEMS 560 microm long and 720 microm wide exhibited a Her2 concentration sensitivity of 5 ng/ml in a background of 1 mg/ml BSA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Line
  • Equipment Design
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Glass
  • Humans
  • Kinetics
  • Lead / chemistry*
  • Organosilicon Compounds
  • Receptor, ErbB-2 / analysis*
  • Receptor, ErbB-2 / biosynthesis
  • Reproducibility of Results
  • Serum Albumin, Bovine / chemistry
  • Silanes / chemistry
  • Titanium / chemistry*

Substances

  • Organosilicon Compounds
  • Silanes
  • lead titanate
  • Serum Albumin, Bovine
  • Lead
  • (3-mercaptopropyl)trimethoxysilane
  • Titanium
  • ERBB2 protein, human
  • Receptor, ErbB-2