Aim: To study the immunomodulatory effects of spleenic cells added by souble IL-15Ralpha on the regulation of melanoma cell growth.
Methods: The spleenic cells of mice were prepared for culture for 48 hours by adding or not adding soluble IL-15Ralpha (sIL-15Ralpha). Then the adherent cells and non- adherent cells were separated. FACS was used to detect the expression of CD4, CD8, B220, CD11c and CD1a; as mentioned above, the four groups (non-adherent cells of spleenic cells, adherent cells of spleenic cells, non-adherent cells of spleenic cells added by sIL-15Ralpha, adherent cells of spleenic cells added by sIL-15Ralpha) were mixed with melanoma cells separately, then injected intraperitoneally into the cells to mice to observe the tumor volume of the mice, control group was only injected intraperitoneally into melanoma cells.
Results: The melanoma growth rate of mice with adherent cells added by sIL-15Ralpha was significantly less than that of the other groups. FACS showed the molecules of this group were possibly dendritic cells.
Conclusion: sIL-15Ralpha added by spleenic cells can make an immunomodulatory effets possibly mainly by the effects of dendritic cells and inhibit the growth of tumor cells.