Abstract
We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and the deacetylation of histone H3 Lys 4 (H3K4) at the centromere. HDAC3 knockdown induced spindle assembly checkpoint activation and sister chromatid dissociation. The depletion of Polo-like kinase 1 (Plk1) or Aurora B restored cohesion in HDAC3-depleted cells. HDAC3 was also required for Shugoshin localization at centromeres. Finally, we show that HDAC3 depletion results in the acetylation of centromeric H3K4, correlated with a loss of dimethylation at the same position. These findings provide a functional link between sister chromatid cohesion and the mitotic "histone code".
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Aurora Kinase B
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Aurora Kinases
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Cell Cycle Proteins / antagonists & inhibitors
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Centromere / metabolism*
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HeLa Cells
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Histone Deacetylase Inhibitors
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Histone Deacetylases / genetics
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Histone Deacetylases / metabolism*
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Histones / chemistry*
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Histones / metabolism*
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Humans
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Lysine / chemistry
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Mitosis
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Phosphorylation
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Polo-Like Kinase 1
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Protein Serine-Threonine Kinases / antagonists & inhibitors
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism
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Proto-Oncogene Proteins / antagonists & inhibitors
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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RNA, Small Interfering / genetics
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Sister Chromatid Exchange / physiology*
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Transfection
Substances
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Cell Cycle Proteins
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Histone Deacetylase Inhibitors
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Histones
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Proto-Oncogene Proteins
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RNA, Small Interfering
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SGO1 protein, human
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AURKB protein, human
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Aurora Kinase B
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Aurora Kinases
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Protein Serine-Threonine Kinases
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Histone Deacetylases
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histone deacetylase 3
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Lysine