A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses

Hanako Shimura; Takako Fukagawa; Ayano Meguro; Hirokazu Yamada; Mahito Oh-Hira; Shinsuke Sano; Chikara Masuta

doi:10.1016/j.febslet.2008.10.046

A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses

FEBS Lett. 2008 Dec 10;582(29):4047-52. doi: 10.1016/j.febslet.2008.10.046. Epub 2008 Nov 7.

Authors

Hanako Shimura¹, Takako Fukagawa, Ayano Meguro, Hirokazu Yamada, Mahito Oh-Hira, Shinsuke Sano, Chikara Masuta

Affiliation

¹ Laboratory of Cell Biology and Manipulation, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.

PMID: 18996375
DOI: 10.1016/j.febslet.2008.10.046

Abstract

To find out whether we can control plant virus diseases by blocking viral RNA silencing suppressors (RSSs), we developed a strategy to screen inhibitors that block the association of RSSs with siRNAs using a surface plasmon resonance assay. The screened chemicals were tested in competition with RSSs for binding to siRNAs using a mobility shift assay. We then confirmed that tested chemicals actually inhibited the RSS activity in vivo using a protoplast assay which was developed for this purpose. This entire system can be adapted to screening inhibitors of not only plant viruses but also some animal viruses possessing RSSs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antiviral Agents / chemistry
Antiviral Agents / isolation & purification*
Antiviral Agents / pharmacology
Drug Discovery
Plant Diseases / virology*
Plant Viruses / drug effects*
Plant Viruses / genetics
RNA Interference / drug effects*
RNA, Small Interfering / drug effects
RNA, Viral / drug effects

Substances

Antiviral Agents
RNA, Small Interfering
RNA, Viral